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Working principle of ultra-micro-volume spectrophotometer

Working principle of ultra-micro-volume spectrophotometer

The ultra-micro-volume spectrophotometer is a high-precision experimental instrument used to measure the absorption and emission spectra of trace substances in a sample. Today we will take a look at the composition and working principle of this instrument.

The ultra-micro spectrophotometer is composed of light source, optical system, detector and computer. First, the light source emits a beam of light that passes through the sample, is then focused by an optical system, and enters a detector for analysis. When compounds are present in a sample, they absorb or emit in specific regions of the spectrum, so a detector measures the intensity of the absorbed or emitted light and converts it into a digital signal. Finally, the computer calculates the concentration of the compound in the sample or other relevant data based on these digital signals.

The main application fields of ultra-micro spectrophotometer include chemistry, biology, pharmacy and environmental science. For example, it could be used to determine the concentration of a certain toxic substance in water, detect the chemical composition of blood plasma, and even explore interstellar matter. Due to its high precision and sensitivity, the micro-volume spectrophotometer has become an important tool for scientific research and laboratory analysis, and is of great significance to both academia and industry.


Post time: Mar-07-2023